Voltage Sensor Movements

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Voltage Sensor Movements

The voltage dependence of K , Na , and Ca 2 channels is brought about by a voltage sensor that moves 12–13 e 0 across the entire electric field (Schoppa et al., 1992; Hirschberg et al., 1996; Noceti et al., 1996). In the case of Shaker K channel it is known which residues are responsible for this large amount of gating charge. This was found by measuring the total gating charge movement per cha...

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Molecular mechanism of voltage sensor movements in a potassium channel.

Voltage-gated potassium channels are six-transmembrane (S1-S6) proteins that form a central pore domain (4 x S5-S6) surrounded by four voltage sensor domains (S1-S4), which detect changes in membrane voltage and control pore opening. Upon depolarization, the S4 segments move outward carrying charged residues across the membrane field, thereby leading to the opening of the pore. The mechanism of...

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Fast and Slow Voltage Sensor Movements in HERG Potassium Channels

HERG encodes an inwardly-rectifying potassium channel that plays an important role in repolarization of the cardiac action potential. Inward rectification of HERG channels results from rapid and voltage-dependent inactivation gating, combined with very slow activation gating. We asked whether the voltage sensor is implicated in the unusual properties of HERG gating: does the voltage sensor move...

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Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent ...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 2002

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.20028660